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mouse kidney fibroblast complete culture medium  (Procell Inc)

 
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    Procell Inc mouse kidney fibroblast complete culture medium
    ( A–D ) WT or S4KO mice were randomly assigned to FA or vehicle according to an established protocol. Kidney samples were obtained from mice on day 14 post FA or vehicle treatment (n=6 per group). (A) Western blot analysis of the expression of SIRT4, CCN2, FN1, COL1A1, COL3A1, E-cadherin, α-SMA, and Tubulin in the kidney of mice. (B) Representative images of Masson’s trichrome staining and Sirius red in kidneys from mice (scale bar, 100 μm). (C, D) The mRNA level of Col1a1, Fn1, Eln, Ccn2, Acta2 , Col3a1, Ngal , and Kim-1 in the kidney of mice. ( E ) RT-PCR (n=5) and western blot analysis (n=3) of the expression of SIRT4 in selected mouse renal cells including mouse podocytes (MPC), glomerular endothelial cells (GEC), mouse TECs and mouse <t>fibroblast</t> (MF). ( F–H ) Control or S4FKO mice were randomly assigned to sham or UUO surgery according to an established protocol. (F) Western blot analysis of the expression of SIRT4, CCN2, FN1, COL1A1, COL3A1, α-SMA, and Tubulin in the kidney of mice. (G) The mRNA level of Col1a1, Fn1, Eln, Ccn2, Acta2 , Col3a1, Ngal , and Kim-1 in the kidney of mice. (H) Representative images of Masson’s trichrome staining and Sirius red in kidney sections of mice (scale bar, 100 μm). For all panels, data are presented as mean ± SD. *p<0.05, **p<0.01, ***p<0.001 by one-way ANOVA with Bonferroni correction test. For all panels, data are presented as mean ± SD. **p<0.01, ***p<0.001 by one-way ANOVA with Bonferroni correction test. Figure 2—figure supplement 1—source data 1. Original files for western blot analysis displayed in . Figure 2—figure supplement 1—source data 2. The uncropped gels or blots with 1109 the relevant bands clearly labeled in .
    Mouse Kidney Fibroblast Complete Culture Medium, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse kidney fibroblast complete culture medium/product/Procell Inc
    Average 90 stars, based on 1 article reviews
    mouse kidney fibroblast complete culture medium - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "Nuclear translocation of SIRT4 mediates deacetylation of U2AF2 to modulate renal fibrosis through alternative splicing-mediated upregulation of CCN2"

    Article Title: Nuclear translocation of SIRT4 mediates deacetylation of U2AF2 to modulate renal fibrosis through alternative splicing-mediated upregulation of CCN2

    Journal: eLife

    doi: 10.7554/eLife.98524

    ( A–D ) WT or S4KO mice were randomly assigned to FA or vehicle according to an established protocol. Kidney samples were obtained from mice on day 14 post FA or vehicle treatment (n=6 per group). (A) Western blot analysis of the expression of SIRT4, CCN2, FN1, COL1A1, COL3A1, E-cadherin, α-SMA, and Tubulin in the kidney of mice. (B) Representative images of Masson’s trichrome staining and Sirius red in kidneys from mice (scale bar, 100 μm). (C, D) The mRNA level of Col1a1, Fn1, Eln, Ccn2, Acta2 , Col3a1, Ngal , and Kim-1 in the kidney of mice. ( E ) RT-PCR (n=5) and western blot analysis (n=3) of the expression of SIRT4 in selected mouse renal cells including mouse podocytes (MPC), glomerular endothelial cells (GEC), mouse TECs and mouse fibroblast (MF). ( F–H ) Control or S4FKO mice were randomly assigned to sham or UUO surgery according to an established protocol. (F) Western blot analysis of the expression of SIRT4, CCN2, FN1, COL1A1, COL3A1, α-SMA, and Tubulin in the kidney of mice. (G) The mRNA level of Col1a1, Fn1, Eln, Ccn2, Acta2 , Col3a1, Ngal , and Kim-1 in the kidney of mice. (H) Representative images of Masson’s trichrome staining and Sirius red in kidney sections of mice (scale bar, 100 μm). For all panels, data are presented as mean ± SD. *p<0.05, **p<0.01, ***p<0.001 by one-way ANOVA with Bonferroni correction test. For all panels, data are presented as mean ± SD. **p<0.01, ***p<0.001 by one-way ANOVA with Bonferroni correction test. Figure 2—figure supplement 1—source data 1. Original files for western blot analysis displayed in . Figure 2—figure supplement 1—source data 2. The uncropped gels or blots with 1109 the relevant bands clearly labeled in .
    Figure Legend Snippet: ( A–D ) WT or S4KO mice were randomly assigned to FA or vehicle according to an established protocol. Kidney samples were obtained from mice on day 14 post FA or vehicle treatment (n=6 per group). (A) Western blot analysis of the expression of SIRT4, CCN2, FN1, COL1A1, COL3A1, E-cadherin, α-SMA, and Tubulin in the kidney of mice. (B) Representative images of Masson’s trichrome staining and Sirius red in kidneys from mice (scale bar, 100 μm). (C, D) The mRNA level of Col1a1, Fn1, Eln, Ccn2, Acta2 , Col3a1, Ngal , and Kim-1 in the kidney of mice. ( E ) RT-PCR (n=5) and western blot analysis (n=3) of the expression of SIRT4 in selected mouse renal cells including mouse podocytes (MPC), glomerular endothelial cells (GEC), mouse TECs and mouse fibroblast (MF). ( F–H ) Control or S4FKO mice were randomly assigned to sham or UUO surgery according to an established protocol. (F) Western blot analysis of the expression of SIRT4, CCN2, FN1, COL1A1, COL3A1, α-SMA, and Tubulin in the kidney of mice. (G) The mRNA level of Col1a1, Fn1, Eln, Ccn2, Acta2 , Col3a1, Ngal , and Kim-1 in the kidney of mice. (H) Representative images of Masson’s trichrome staining and Sirius red in kidney sections of mice (scale bar, 100 μm). For all panels, data are presented as mean ± SD. *p<0.05, **p<0.01, ***p<0.001 by one-way ANOVA with Bonferroni correction test. For all panels, data are presented as mean ± SD. **p<0.01, ***p<0.001 by one-way ANOVA with Bonferroni correction test. Figure 2—figure supplement 1—source data 1. Original files for western blot analysis displayed in . Figure 2—figure supplement 1—source data 2. The uncropped gels or blots with 1109 the relevant bands clearly labeled in .

    Techniques Used: Western Blot, Expressing, Staining, Reverse Transcription Polymerase Chain Reaction, Control, Labeling



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    mouse kidney fibroblast complete culture medium  (Procell Inc)
    90
    Procell Inc mouse kidney fibroblast complete culture medium
    ( A–D ) WT or S4KO mice were randomly assigned to FA or vehicle according to an established protocol. Kidney samples were obtained from mice on day 14 post FA or vehicle treatment (n=6 per group). (A) Western blot analysis of the expression of SIRT4, CCN2, FN1, COL1A1, COL3A1, E-cadherin, α-SMA, and Tubulin in the kidney of mice. (B) Representative images of Masson’s trichrome staining and Sirius red in kidneys from mice (scale bar, 100 μm). (C, D) The mRNA level of Col1a1, Fn1, Eln, Ccn2, Acta2 , Col3a1, Ngal , and Kim-1 in the kidney of mice. ( E ) RT-PCR (n=5) and western blot analysis (n=3) of the expression of SIRT4 in selected mouse renal cells including mouse podocytes (MPC), glomerular endothelial cells (GEC), mouse TECs and mouse <t>fibroblast</t> (MF). ( F–H ) Control or S4FKO mice were randomly assigned to sham or UUO surgery according to an established protocol. (F) Western blot analysis of the expression of SIRT4, CCN2, FN1, COL1A1, COL3A1, α-SMA, and Tubulin in the kidney of mice. (G) The mRNA level of Col1a1, Fn1, Eln, Ccn2, Acta2 , Col3a1, Ngal , and Kim-1 in the kidney of mice. (H) Representative images of Masson’s trichrome staining and Sirius red in kidney sections of mice (scale bar, 100 μm). For all panels, data are presented as mean ± SD. *p<0.05, **p<0.01, ***p<0.001 by one-way ANOVA with Bonferroni correction test. For all panels, data are presented as mean ± SD. **p<0.01, ***p<0.001 by one-way ANOVA with Bonferroni correction test. Figure 2—figure supplement 1—source data 1. Original files for western blot analysis displayed in . Figure 2—figure supplement 1—source data 2. The uncropped gels or blots with 1109 the relevant bands clearly labeled in .
    Mouse Kidney Fibroblast Complete Culture Medium, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse kidney fibroblast complete culture medium/product/Procell Inc
    Average 90 stars, based on 1 article reviews
    mouse kidney fibroblast complete culture medium - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A–D ) WT or S4KO mice were randomly assigned to FA or vehicle according to an established protocol. Kidney samples were obtained from mice on day 14 post FA or vehicle treatment (n=6 per group). (A) Western blot analysis of the expression of SIRT4, CCN2, FN1, COL1A1, COL3A1, E-cadherin, α-SMA, and Tubulin in the kidney of mice. (B) Representative images of Masson’s trichrome staining and Sirius red in kidneys from mice (scale bar, 100 μm). (C, D) The mRNA level of Col1a1, Fn1, Eln, Ccn2, Acta2 , Col3a1, Ngal , and Kim-1 in the kidney of mice. ( E ) RT-PCR (n=5) and western blot analysis (n=3) of the expression of SIRT4 in selected mouse renal cells including mouse podocytes (MPC), glomerular endothelial cells (GEC), mouse TECs and mouse fibroblast (MF). ( F–H ) Control or S4FKO mice were randomly assigned to sham or UUO surgery according to an established protocol. (F) Western blot analysis of the expression of SIRT4, CCN2, FN1, COL1A1, COL3A1, α-SMA, and Tubulin in the kidney of mice. (G) The mRNA level of Col1a1, Fn1, Eln, Ccn2, Acta2 , Col3a1, Ngal , and Kim-1 in the kidney of mice. (H) Representative images of Masson’s trichrome staining and Sirius red in kidney sections of mice (scale bar, 100 μm). For all panels, data are presented as mean ± SD. *p<0.05, **p<0.01, ***p<0.001 by one-way ANOVA with Bonferroni correction test. For all panels, data are presented as mean ± SD. **p<0.01, ***p<0.001 by one-way ANOVA with Bonferroni correction test. Figure 2—figure supplement 1—source data 1. Original files for western blot analysis displayed in . Figure 2—figure supplement 1—source data 2. The uncropped gels or blots with 1109 the relevant bands clearly labeled in .

    Journal: eLife

    Article Title: Nuclear translocation of SIRT4 mediates deacetylation of U2AF2 to modulate renal fibrosis through alternative splicing-mediated upregulation of CCN2

    doi: 10.7554/eLife.98524

    Figure Lengend Snippet: ( A–D ) WT or S4KO mice were randomly assigned to FA or vehicle according to an established protocol. Kidney samples were obtained from mice on day 14 post FA or vehicle treatment (n=6 per group). (A) Western blot analysis of the expression of SIRT4, CCN2, FN1, COL1A1, COL3A1, E-cadherin, α-SMA, and Tubulin in the kidney of mice. (B) Representative images of Masson’s trichrome staining and Sirius red in kidneys from mice (scale bar, 100 μm). (C, D) The mRNA level of Col1a1, Fn1, Eln, Ccn2, Acta2 , Col3a1, Ngal , and Kim-1 in the kidney of mice. ( E ) RT-PCR (n=5) and western blot analysis (n=3) of the expression of SIRT4 in selected mouse renal cells including mouse podocytes (MPC), glomerular endothelial cells (GEC), mouse TECs and mouse fibroblast (MF). ( F–H ) Control or S4FKO mice were randomly assigned to sham or UUO surgery according to an established protocol. (F) Western blot analysis of the expression of SIRT4, CCN2, FN1, COL1A1, COL3A1, α-SMA, and Tubulin in the kidney of mice. (G) The mRNA level of Col1a1, Fn1, Eln, Ccn2, Acta2 , Col3a1, Ngal , and Kim-1 in the kidney of mice. (H) Representative images of Masson’s trichrome staining and Sirius red in kidney sections of mice (scale bar, 100 μm). For all panels, data are presented as mean ± SD. *p<0.05, **p<0.01, ***p<0.001 by one-way ANOVA with Bonferroni correction test. For all panels, data are presented as mean ± SD. **p<0.01, ***p<0.001 by one-way ANOVA with Bonferroni correction test. Figure 2—figure supplement 1—source data 1. Original files for western blot analysis displayed in . Figure 2—figure supplement 1—source data 2. The uncropped gels or blots with 1109 the relevant bands clearly labeled in .

    Article Snippet: Mouse renal fibroblasts (MF; Procell Life Science&Technology, Wuhan, china; Cat# CP-M069) were cultured in mouse kidney fibroblast complete culture medium (Procell, Cat#CM-M069).

    Techniques: Western Blot, Expressing, Staining, Reverse Transcription Polymerase Chain Reaction, Control, Labeling